Chemical reagents are made up of several components that interact with the analyte present in the sample, so generating a chemical reaction.
Enzymatic reagents contain several components (enzymes, substrates, cofactors ...) that interact specifically with the analyte present in the sample, so generating a biochemical reaction.
The transformation or combination thereof results in the formation of product with properties, characteristics and / or different conformations that generate a signal measurable. spectrophotometrically and proportional to the amount of analyte present.
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